FIGURE 3.
The effects of Gar1 on substrate RNA docking. (A) Fluorescence intensity profile of substrate RNA bound with guide RNA (gRNA),Cbf5, and Nop10 (CN RNP) when Gar1 was added before L7Ae. (B) Fluorescence intensity profile of substrate RNA bound with guide RNA,Cbf5, and Nop10 (CN RNP) when Gar1 was added after L7Ae.; (C) the same titration as of A but with Cbf5 Δloop mutation; (D) the same titration order as of B but with Cbf5 Δloop mutation. (E) Previously observed interactions between Gar1 and the β7/β10 loop of Cbf5. The coordinates are from crystal structure of the P. furiosus Cbf5–Nop10–Gar1 complex (PDB code 2EY4).
