FIGURE 1.
Schematic H/ACA RNP assembly and placement of a fluorescence label on the substrate RNA. (A) Composition and organization of box H/ACA RNPs. “Ψ” denotes the target uridine, which is to be converted to pseudouridine, and it is replaced by 5-floururidine (5-FU) unless noted otherwise. “N” denotes the nucleotide 3′ to the target uridine that is replaced by 2-aminopurine (2-AP). (B) An illustration of proposed changes in fluorescence intensities due to the conformation of the substrate RNA. The position of 2-AP is indicated. Docking of the substrate RNA leads to unstacking of 2-AP, thereby, high fluorescence intensity.
