FIGURE 4.
Binding of hnRNP LL to ESS1 increases relative to hnRNP L upon cellular stimulation. (A) Western blot of hnRNP L and LL following affinity purification (“pull-down”) with biotinylated ESS1, mutant (mESS) or nonspecific (NS) 60-mer RNAs from JSL1 nuclear extract (NE) prepared from resting (−PMA) or activated (+PMA) cells. (B) UV cross-linking experiments done with uniformly labeled ESS1 or mESS RNA and nuclear extract from resting or stimulated cells. As indicated, parallel cross-linked samples were subject to immunoprecipitation with antibodies to hnRNP L, LL, or an IgG control prior to running of the SDS-PAGE.
