Toward predicting self-splicing and protein-facilitated splicing of group I introns

Facilitation or inhibition of splicing upon addition of the CYT-18 protein. (A) Time course self-splicing experiment (5, 20, and 60 min time points) of the S.h. intron in the presence of increasing concentrations of the CYT-18 protein (onefold and fivefold of the RNA concentration). (B) Comparison of the effects of the addition of full length CYT-18 or of C-terminally truncated CYT-18 on the self-splicing activity of the N.c., S.h., and A.p.LSU introns. (C) Product ratios obtained in the presence of full length or C-terminally truncated CYT-18 protein. The error bars indicate the standard deviation calculated from three independent experiments.