The sarcin–ricin loop of 23S rRNA is essential for assembly of the functional core of the 50S ribosomal subunit

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FIGURE 5.
FIGURE 5.

ΔSRL 50S subunits are missing ribosomal protein L16. (A) SDS gel of proteins extracted from affinity-purified WT and ΔSRL 50S subunits; arrow indicates the missing protein band. (B) Two-dimensional protein gel of WT and ΔSRL 50S subunits; protein L16 is circled. (C) Denaturing gel of rRNA extracted from 50S subunits shows that 5S rRNA is present in normal amounts in ΔSRL subunits.

This Article

  1. Published in Advance August 28, 2008, doi: 10.1261/rna.1202108 RNA 14: 1999-2012

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