The sarcin–ricin loop of 23S rRNA is essential for assembly of the functional core of the 50S ribosomal subunit

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FIGURE 2.
FIGURE 2.

ΔSRL 50S subunits are inactive in protein synthesis, but can associate with 30S subunits. (A) Poly-U mRNA directed [14C]-Phe incorporation was measured for ribosomes containing WT (■) or ΔSRL (▼) 50S subunits. Sucrose density-gradient sedimentation of WT and ΔSRL 50S subunits in the absence (B) or presence (C) of 30S subunits. Arrows indicate the position of 30S subunits, 50S subunits, or 70S ribosomes.

This Article

  1. Published in Advance August 28, 2008, doi: 10.1261/rna.1202108 RNA 14: 1999-2012

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