FIGURE 3.
Electropherogram analysis as implemented using ShapeFinder tools. (A) Unprocessed capillary electrophoresis electropherogram. (B) Net result after application of the Fitted Baseline Adjust and Matrixing tools. (C) After the Mobility Shift: Polynomial tool (from four serial executions of the tool). (D) After signal decay correction and rescaling. (E) Whole-channel Gaussian integration of the (+) and (−) reagent channels obtained using the Align and Integrate tool. (Solid bars) Absolute SHAPE reactivities after subtracting background. For clarity, sequencing channels are offset from the (+) and (−) reagent channels.
