FIGURE 3.
Poly(A) analysis of the products. (A) RNaseH/oligo(dT) reactions. RNAs produced by each tethered protein, prepared as in Figure 2B, were treated with oligo(dT) and RNaseH and analyzed by electrophoresis and autoradiography. (−) No DNA oligo added; (+) reactions included oligo(dT)18. (B) Binding to oligo(dT). RNAs were fractionated using biotinylated oligo(dT). (−) RNA that did not bind to biotinylated oligo(dT); (+) RNA that did bind.
