FIGURE 2.
RNAs are elongated by proteins that do not stimulate translation. (A) Protocol. A radiolabeled RNA containing three MS2 binding sites was injected into oocytes that were expressing a fusion protein. (B) Results. The radiolabeled RNA substrates were analyzed by electrophoresis through urea–polyacrylamide gels. The products are grouped by what proved to be their enzymatic activities (PAPs [lanes 2–6], PUPs [lanes 7–13], or inactive [lanes 14–18]). RNAs from two oocytes were loaded on each lane. (Lane 1) An uninjected input RNA. Markers on left indicate approximate sizes of tails.
