Characterization of a cyanobacterial RNase P ribozyme recognition motif in the IRES of foot-and-mouth disease virus reveals a unique structural element

Time course of the ribozyme reaction with FMDV IRES substrates. Transcripts IRES (A) and 3_86–299 (B) were incubated with the Rz 6803 under optimal conditions; aliquots were withdrawn at the indicated times and fractionated on denaturing gels. The efficiency of cleavage was determined as the percent of label measured in a PhosphorImager in the product of interest relative to the total input normalized to the size of the transcript in each case.