Mapping of the ribozyme cleavage products. Transcripts IRES (A), 386–299 (B), and 3121–261 (C) were labeled either uniformly or at the 3′ end using pCp and T4 RNA ligase prior to its incubation with the Rz. The reaction products were fractionated in denaturing gels. Arrows depict digestion products, while thin lines denote the RNA size markers position. (D) Alignment of the digestion products according to their relative orientation. Absence of a specific product detected in each pCp-labeled RNA relative to the uniformly labeled counterpart was taken as evidence of its 5′ position within the transcript under study. The size of each fragment is indicated in a number of nucleotides.
