A case for “StopGo”: Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)

Analysis of PnPV virion protein digests by FT-ICR. The single 2986 amino acid ORF of PnPV is depicted in sections. Underlined sequences were identified in tryptic or chymotryptic digests of virions. The two 2A sequences are italicized and the most crucial residues for “StopGo” are shown in white with black shading. Putative viral protease recognition sequences are shaded in gray. “pS ” indicates phosphorylation on S ₁₁₈₇. The bold “Y” is the residue expected to link the putative VPg to the 5′ end of PnPV RNA.