New photoreactive tRNA derivatives for probing the peptidyl transferase center of the ribosome

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FIGURE 5.
FIGURE 5.

Analysis of the distribution of cross-links between 23S rRNA and 50S-subunit proteins. (A) 32P-labeled material isolated from 50S subunits was resuspended in 6 M urea, resolved by 4%–20% gradient SDS-PAGE, and visualized by autoradiography. With the exception of unmodified tRNAPhe, the ligands cross-linked to both 23S rRNA and to proteins. (B) Bar graph showing the relative distribution of cross-linked 23S rRNA and protein estimated by PhosphorImager analysis of the gels. Error bars represent the standard deviation in measurements obtained from three separate SDS-PAGE runs.

This Article

  1. Published in Advance March 22, 2007, doi: 10.1261/rna.425907 RNA 13: 793-800

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