New photoreactive tRNA derivatives for probing the peptidyl transferase center of the ribosome

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FIGURE 1.
FIGURE 1.

Synthetic schemes and structures of photoreactive nucleotides and modified tRNA ligands. (A) Synthesis of 2-azido-2′-O-methyladenosine 3′,5′-bisphosphate (p2N32′OMeAp). (B) Synthesis of 2-azido-2′-deoxyadenosine 3′,5′-bisphosphate (p2N3dAp) and its final incorporation into the photoreactive transition-state analog (2N3dA76)tRNAPhe-p-Puro.

This Article

  1. Published in Advance March 22, 2007, doi: 10.1261/rna.425907 RNA 13: 793-800

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