mNoc-induced transcript has a short half-life and is stabilized by cycloheximide. (A) Northern blot showing the mNoc mRNA levels after actinomycin D addition. Quiescent NIH3T3 cells were TPA stimulated as in Fig 2; after 2 h, transcription was inhibited by the addition of actinomycin D. (B) Quantification of the transcript decay rate and effect of CHX on mNoc mRNA half-life. Control NIH3T3 cultures were processed as in A; CHX-treated cells received 10 μM of this translation inhibitor 1 h before TPA stimulation. Values are the mean ± SEM (n = 4/group). mNoc mRNA half-life was estimated to be ∼50 min (dotted line). The effect of the CHX on mRNA half-life was statistically significant (F = 123.00; p = 7.68E-15 by ANOVA). (*) p < 0.05, (**) p < 0.001 by post hoc comparisons.
