FIGURE 3.
mNoc is an immediate early gene (IEG). Quiescent and serum-starved NIH3T3 cells were treated with 10 μM cycloheximide (CHX) 1 h before induction of mNoc with 100 nM TPA as was described in Fig. 2. This treatment showed that TPA acute induction is independent of protein synthesis; thus, mNoc is an IEG. Results are the mean ± SEM of three independent experiments by triplicate (n = 9/group). The effect of the CHX was statistically significant (F = 145, p = 9.11E-25 by ANOVA). (*) p < 0.05, (**) p < 0.01, (***) p < 0.001 by post hoc comparisons.
