FIGURE 2.
Acute induction of mNoc in NIH3T3 cultures. 100 nM TPA (A) or 50% FBS-DMEM (B) produced approximately an eightfold increment in mNoc mRNA levels in quiescent and serum-starved cells. TPA also induced the clock gene mPer1. Confluent cultures were maintained for 2 d in 1% FBS-DMEM before the treatment. Relative transcript levels were determined by Northern blot, quantified by densitometry, and normalized by rRNA content. Values represent the mean ± SEM of three independent experiments in A (n = 8–9/group, F = 10.4, p = 1.3E-12 by ANOVA) and two in B (n = 5/group, F = 15.4, p = 1.8E-10 by ANOVA). (*) p < 0.01, (**) p < 0.001 by post hoc comparisons.
