FIGURE 2.
Effect of KREPB4 repression on editosome integrity. Western analyses and adenylation assays (KREL1* and KREL2*) of glycerol gradient fractions (numbered from top to bottom) from whole cell lysates of KREPB4 RNAi cells grown in the absence (expressed) or presence (repressed) of tet for 3 and 6 d are shown. A fraction that contains ∼20S editosomes was used as a positive control (+). The Western analyses used a cocktail of four MAbs specific for core editosome proteins as indicated, and the blots were stripped and reprobed with MRP1 and RBP16 PAbs and HSP70 MAbs, the latter serving as a loading control.
