Bunyavirus mRNA synthesis is coupled to translation to prevent premature transcription termination

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 4.
FIGURE 4.

T1-UP termination ability is influenced by position of the upstream stop codon. (A) Model BUNV segment BUN-T697 was modified by inserting a stop codon 121 nt upstream to generate BUN-T-STOP. (B) Direct analysis of RNAs synthesized by BUN-T697 and BUN-T-STOP using agarose-urea gel electrophoresis showed T1-UP activity was increased when ribosome translocation was blocked upstream.

This Article

  1. Published in Advance March 30, 2007, doi: 10.1261/rna.436607 RNA 13: 731-736

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA