Bunyavirus mRNA synthesis is coupled to translation to prevent premature transcription termination

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 2.
FIGURE 2.

The BUNV transcription termination signal is nonfunctional when positioned within an ORF. (A) Schematic of pBUN-S(ren) and the RNAs expressed by the model BUNV segment it encodes. (B) Schematic of model BUNV segment BUN-T-BsrGI, which contains the authentic termination signal (T1) and a duplicate termination signal within the coding region (T1-UP). (C) Direct analysis of RNA synthesized by BUN-S(ren) and BUN-T-BsrGI using agarose-urea gel electrophoresis shows the internal T1-UP signal is inactive.

This Article

  1. Published in Advance March 30, 2007, doi: 10.1261/rna.436607 RNA 13: 731-736

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA