Double-stranded regions are essential design components of potent inhibitors of RISC function

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FIGURE 3.
FIGURE 3.

Inhibitor flanking sequence composition affects inhibitor functionality. (A) Schematic showing inhibitor designs containing various flanking compositions: unstructured poly-pyrimidine (16Y + RC + 16Y), arbitrary (16A + RC + 16A), and reverse complement (16 + RC + 16), around an antisense core. (B) These inhibitor designs were tested for the ability to inhibit let-7c (white bars) or miR-21 (black bars) at concentrations of 25 and 50 nM. No-inhibitor control is shown (Inhibitor [−]).

This Article

  1. Published in Advance March 30, 2007, doi: 10.1261/rna.448107 RNA 13: 723-730

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