Double-stranded regions are essential design components of potent inhibitors of RISC function

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FIGURE 2.
FIGURE 2.

Overall length of 2′-O-methylated miRNA inhibitors affects functionality. (A) Schematic showing 2′-O-methyl-modified inhibitors with central region reverse complement to mature (dark bar) and flanking regions (reverse complement to pri-miRNA) with lengths varying from 0 to 16 nt on each side. Inhibitors targeting let-7c (B) and miR-21 (C) were assayed by cotransfecting into HeLa cells at 25 (open circles) and 50 nM (filled circles) concentrations. The dual luciferase ratio was measured 48 h post-transfection to determine the effectiveness of each design.

This Article

  1. Published in Advance March 30, 2007, doi: 10.1261/rna.448107 RNA 13: 723-730

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