Structure probing of tmRNA in distinct stages of trans-translation

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FIGURE 4.
FIGURE 4.

Trans-translation steps. Ribosomal complexes (RCs) were prepared and purified as described in Materials and Methods. (RC0) ribosomes contain MFTI-tRNAIle in the P site and an empty A site; (RCI) ribosomes contain MFTI-tRNAIle in the P site and Ala-tmRNA•SmpB•Tu•GDP complex stalled by the antibiotic kirromycin in the A site; (RCII) ribosomes contain deacylated tRNAIle in the P site and MFTIA-tmRNA in the A site; (RCIII) ribosomes contain deacylated tRNAIle in the E site, MFTIA-tmRNA in the P site, and the resume codon of tmRNAs internal ORF in the A site; (RCIV) ribosomes contain deacylated tmRNA in the E site, MFTIAA-tRNAAla in the P site and an empty A site containing the second codon of tmRNA's internal ORF.

This Article

  1. Published in Advance March 30, 2007, doi: 10.1261/rna.451507 RNA 13: 713-722

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