Weak binding affinity of human 4EHP for mRNA cap analogs

Amino acid alignment of human eIF4E (gi:4503535) with 4EHP (gi:3172339) performed with CLUSTALW (Thompson et al. 1994). Residues that are identical in proteins are shadowed in black and conserved substitutions in gray. The conserved residues that play a role in cap binding by eIF4E (Marcotrigiano et al. 1997), with changes in 4EHP, are labeled above. Stars below the lines indicate the positions of eight evolutionarily conserved tryptophan residues in eIF4E.