Combining SELEX and the yeast three-hybrid system for in vivo selection and classification of RNA aptamers

Three-hybrid assay with representative members from all classes. (A) HIS3 expression was monitored as growth on selection plates (SC −his+5 mM 3-AT). Strain L40-coat expressed either Rrm4^AD-G (top) or IRP1^AD-G (bottom). The respective hybrid RNA is indicated next to the various plate sectors. (B) β-galactosidase expression was quantified using a fluorogenic substrate. The difference in relative fluorescence units (RFUs) per minute is given for strains expressing the different proteins (inlay) and hybrid RNAs (bottom) tested.