Combining SELEX and the yeast three-hybrid system for in vivo selection and classification of RNA aptamers

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FIGURE 1.
FIGURE 1.

Course of the SELEX experiment using recombinant Rrm4GST as a target. (A) SDS-PAGE analysis of recombinant Rrm4GST preparations after staining with Coomassie brilliant blue. The full-length fusion protein (111 kDa) and putative degradation products (asterisks) are indicated. (B) Graphical representation of the percentages of bound RNA for different cycles of SELEX. DNA from the 11th cycle (asterisk) was cloned and 49 clones were sequenced.

This Article

  1. Published in Advance February 5, 2007, doi: 10.1261/rna.334307 RNA 13: 614-622

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