Functional defects in transfer RNAs lead to the accumulation of ribosomal RNA precursors

Processing of rRNA in strains grown under slow growth conditions. (A) Strains CJ1891 and CJ1892 were grown to midlog phase in minimal M9-glucose medium at 37°C or in LB medium at 16°C, followed by RNA isolation and primer extension analysis of 23S rRNA. The P/M ratio for each RNA sample is noted above the gel. (Lanes 1,3) CJ1891; (lanes 2,4) CJ1892. (B) Strains CJ1891 and CJ1892 were grown on a minimal M9-glucose plate at 37°C for 2 d or on an LB plate at 16°C for 1 wk.