A novel family of C. elegans snRNPs contains proteins associated with trans-splicing

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FIGURE 5.
FIGURE 5.

Association of snRNAs and proteins demonstrated by gradient mobility shift assays. Wild-type embryonic extracts were fractionated on 10%–40% glycerol gradients without addition of antibody serum (A) or preincubated with antibodies against SL21p (B) or SL26p (C). Each fraction was divided for RNA and protein analysis. Northern blots were probed for various snRNAs and Western blots probed for SL21p and SL26p. Arrows indicate the approximate sedimentation peak fraction of chicken ovalbumin (∼44 kDa), β amylase (∼200 kDa), and apoferritin (∼443 kDa) run in a parallel gradient.

This Article

  1. Published in Advance February 5, 2007, doi: 10.1261/rna.426707 RNA 13: 511-520

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