Benomyl treatment of cells does not alter mRNA decay or global translation. rpb1-1 cells were treated with either benomyl (A) or DMSO alone (B), and the half-life of MFA2 mRNA was determined by transcriptional shut-off by temperature shift. RNA was isolated at various time points after temperature shift (indicated in the figure) and analyzed by Northern blot. Cells containing a chromosomal copy of TUB1-GFP were treated with either benomyl (C) or DMSO alone (D), and sucrose gradient fractionation followed by polysome analysis was carried out as described in the Materials and Methods. Curves represent a continuous plot of absorbance at 254 nm (y-axis) versus position in the sucrose gradient (x-axis). 40S and 60S ribosomal subunit peaks as well as 80S monosome peaks, polysome peaks, and RNP peaks are labeled on the curves.
