Both benomyl and tub1-724-mediated microtubule disruption leads to the formation of Dcp2p-containing foci. Cultured cells encoding a chromosomal copy of TUB1-GFP transformed with DCP2-RFP encoded on a plasmid were treated with DMSO alone (A) or benomyl (B), followed by imaging of the cells by fluorescence microscopy. The two sets of panels per treatment represent different images from the same preparation. (C) TUB1 and tub1-724 cell cultures were transformed with DCP2-GFP encoded on a plasmid, and were grown at either the permissive temperature (30°C) or the restrictive temperature (15°C), followed immediately by imaging of the cells by fluorescence microscopy.
