FIGURE 2.
Our five classes of 22 mixing matrices (MM) for generating diverse sequence pools. The matrix classes are developed based on alteration of diagonal elements (class A) and covariance mutations (classes B–E). For pool synthesis using four vials, the mixing matrix is a 4×4 matrix specifying the molar fractions of nucleotide components A, C, G, and U in the four vials. The columns represent the molar fraction of the four bases in vial for each base denoted in each row.
