Gene silencing effect of siRNA conjugated with membrane permeant peptides (MPPs). (A) Conjugation of siRNA with two different MPPs: penetratin and transportan. MPPs and siRNAs were synthesized with a thiol group attached. The reaction between the thiol groups on the siRNAs and the MPPs was catalyzed by the oxidant diamide. (B) Silencing effect of siRNA on luciferase activity. CHO-AA8-Luc Tet-Off cells stably expressing luciferase were transfected with 25 nM siRNA conjugated with MPPs (penetratin and transportan) or complexed with Lipofectamine 2000. Luciferase activity was measured at days 1, 2, and 3 post-transfection. Reproduced with permission from Muratovska and Eccles (2004).
