mRNA fold change increases multiplicatively with extended seed match count. (A) For miR-1 (open circles) and miR-124 (solid black circles), the total number of extended seed matches was enumerated for each mRNA, and the mean nLFCs in the Lim transfection experiments were determined for sets of mRNAs grouped by seed match count (set sizes indicated above or below points). (Solid lines) Least squares fit for the whole data set. Error bars correspond to standard error. For each of these plots, the proportions of different seed match types for different seed match counts remained fairly constant. (B) Same as A for miR-430 extended seed match counts following Dicer knockout in zebrafish embryos (Giraldez et al. 2006). (C) Same as A for conserved extended seed match counts to 31 “responsive” mouse miRNAs (see Supplemental Material) in CDKO MEFs.
