FIGURE 5.
Small molecules that induce exon skipping do not function as PMA mimics. (A) Western blot for Erk expression in cells treated with the compounds shown. Cells were harvested at 15, 20, 30, and 60 min after treatment with the compounds, since Erk phosphorylation is a rapid and transient response to stimulation. (B) Flow cytometry analysis of CD69 surface expression in cells treated with PMA, CRS 3–248 or XJB 1–255 (lines) versus DMSO control (filled). Cells were harvested, washed with PBS, incubated with PE-conjugated anti-CD69 and analyzed for PE fluorescence after gating on live cells.
