Use of transcriptional synergy to augment sensitivity of a splicing reporter assay

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FIGURE 3.
FIGURE 3.

Small molecules that induce GFP expression in reporter cells also induce exon skipping. (A) RT-PCR analysis of the isoform expression of the pSC-VP16 minigene in cells grown for 72 h with DMSO or the small molecules listed. (B) Average change in splicing from two to four independent experiments. Quantitation was done as described in Figure 1.

This Article

  1. Published in Advance March 23, 2006, doi: 10.1261/rna.8306 RNA 12: 925-930

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