FLIPing heterokaryons to analyze nucleo-cytoplasmic shuttling of yeast proteins

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FIGURE 1.
FIGURE 1.

Heterokaryon assay. Cells expressing either Arx1p–GFP or Gar1p–GFP were mated with kar1-1 strain and placed on YPD plate containing cycloheximide to inhibit de novo protein synthesis. After a 2-h incubation, heterokaryons were analyzed by fluorescence microscopy for presence of GFP signal in both nuclei.

This Article

  1. Published in Advance March 15, 2006, doi: 10.1261/rna.2301806 RNA 12: 921-930

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