Enrichment and analysis of RNA centered on ion pair reverse phase methodology

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FIGURE 4.
FIGURE 4.

IP RP μHPLC chromatogram of the enriched small RNAs from total RNA spiked with synthetic let-7 miRNA. The chromatogram shows the analysis of the small RNA fraction obtained following the solid phase enrichment of a total RNA (20 μg) spiked with synthetic miRNA (let-7 150 ng) using gradient 5 (see Materials and Methods). The let-7 miRNA species is highlighted.

This Article

  1. Published in Advance February 22, 2006, doi: 10.1261/rna.2278606 RNA 12: 691-696

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