Enrichment and analysis of RNA centered on ion pair reverse phase methodology

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FIGURE 1.
FIGURE 1.

IP RP HPLC chromatogram of eukaryotic total RNA. The chromatogram shows the analysis of (3 μg) total RNA extracted from Hela cells using gradient 2 (see Materials and Methods). A shows the typical profile of the early eluting small RNA species and the later coeluting 18S and 23S rRNA. B shows an enhanced view of the smaller RNA species. The tRNA, 5.8S, and 5S rRNA are highlighted.

This Article

  1. Published in Advance February 22, 2006, doi: 10.1261/rna.2278606 RNA 12: 691-696

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