Sulfolobus solfataricus translation initiation factor 1 stimulates translation initiation complex formation

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 2.
FIGURE 2.

aIF1 is localized on the 30S ribosomal subunit. (A) Sedimentation profile (10%–30% sucrose gradient) of a S. solfataricus cell lysate, determined by A260 measurement in the absence of added mRNA. (B) Sedimentation profile (10%–30% sucrose gradient) of a S. solfataricus cell lysate programmed with 104 mRNA upon treatment with formaldehyde. The top and the bottom of the gradient are indicated. The gradients were fractionated and the fractions were probed for the presence of aIF1 with polyclonal antibodies raised against recombinant S. solfataricus aIF1 as described in Materials and Methods. The corresponding Western blots are shown below the sedimentation profiles.

This Article

  1. Published in Advance March 3, 2006, doi: 10.1261/rna.2289306 RNA 12: 674-682

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA