Assignment of the transcriptional start site, the correct amino acid sequence and expression of the aIF1 gene in vitro. (A) Amino acid sequence of aIF1 (affected amino acids are underlined) and the corrected DNA subsequence (the numbers correspond to the S. solfataricus genome coordinates [Singh et al. 2004]) of the aIF1 gene. Sequence errors in the published aIF1 sequence are underlined. (B) Determination of the 5′-end of aIF1 mRNA by primer extension. The primer extension signal (PE) corresponds to the G nucleotide, 4 nt upstream of the AUG start codon. (C) In vitro translation of aIF1 mRNA using a S. solfataricus in vitro translation system. The position of the aIF1 protein is indicated by an arrow, and the positions of molecular weight markers are shown at the left.
