The La protein functions redundantly with tRNA modification enzymes to ensure tRNA structural stability

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FIGURE 1.
FIGURE 1.

A mutation in RRS1 causes yeast to require LHP1 for efficient aminoacylation of tRNAArgCCG. (A) Fivefold serial dilutions of wild-type cells (RRS1 LHP1), cells containing a mutation in the arginyl-tRNA synthetase (rrs1-100 LHP1), cells lacking LHP1 (RRS1 lhp1Δ), and rrs1-100 cells lacking LHP1 (rrs1-100 lhp1Δ) were spotted onto YPD agar and grown at the indicated temperatures. (B) Fivefold serial dilutions of wild-type cells (RRS LHP1), rrs1-100 LHP1 cells carrying either TRR4 on the centromeric plasmid pRS316 (pTRR4) or the pRS316 plasmid alone (vector), and rrs1-100 lhp1Δ cells carrying either pTRR4 or pRS316 were spotted on YPD agar and grown at 16°C (left) or 25°C (right). (C) RNA from wild-type (RRS LHP1), rrs1-100 (rrs1-100 LHP1), lhp1Δ (RRS1 lhp1Δ), and rrs1-100 lhp1Δ cells grown at 25°C was extracted under acidic conditions and fractionated in acidic acrylamide gels. The blot was probed to detect tRNAArgCCG (Arg4), tRNAArgCCU (Arg1), tRNAArgACG (Arg2), tRNAArgUCU (Arg3), and tRNAThrCGU.

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  1. RNA 12: 644-654

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