Characteristics of the glmS ribozyme suggest only structural roles for divalent metal ions

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FIGURE 4.
FIGURE 4.

Cation requirements for self-cleavage of the B. cereus 201 glmS ribozyme. (A) Effect on the rate constant of substituting Mg2+ with varying concentrations of Mn2+ or Ca2+. (B) Effect on the rate constant of varying concentrations of Co(NH3)6Cl3. (C) Cleavage activity in high concentrations of KCl. Reactions in B and in C were performed in the absence of added divalent metal ions and supplemented with EDTA at concentrations of 2.5 mM and 25 mM, respectively.

This Article

  1. Published in Advance February 16, 2006, doi: 10.1261/rna.2266506 RNA 12: 607-619

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