TABLE 1.
Folding parameters for the I-to-N transition obtained by CD
| S-domain | No. of nt changes | KMg (mM) | n | m (kcal mol−1 m−1) |
|---|---|---|---|---|
| 20 mM TrisHCl (pH 8.1), 37°C. | ||||
| E. coli | 0 | 0.15 ± 0.01 | 3.9 ± 0.5 | 1.5 ± 0.1 |
| T. thermophilus | 39 | 0.08 ± 0.01 | 6.2 ± 0.6 | 2.4 ± 0.2 |
| Mutants | ||||
| P12 | 2 | 0.17 ± 0.01 | 3.0 ± 0.6 | |
| P9 + P11 | 4 | 0.07 ± 0.02 | 3.8 ± 1.7 | |
| J11/12 + P14 | 6 | 0.09 ± 0.01 | 2.9 ± 0.7 | |
| All 12 | 12 | 0.09 ± 0.01 | 6.4 ± 1.6 | |
