Structural basis for altering the stability of homologous RNAs from a mesophilic and a thermophilic bacterium

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 2.
FIGURE 2.

Folding of E. coli (□) and T. thermophilus (▪) S-domains monitored by CD (top panel) and absorbance (bottom panel) at 287 nm (0.3 μM RNA, 37°C, 20 mM Tris at pH 8.1). The inset shows a plot for titrations performed at various [urea] to determine the m-value. Changes in absorbance and CD track the U-to-I transition and the I-to-N transition, respectively.

This Article

  1. RNA 12: 598-606