FIGURE 1.
Northern blot hybridization analysis of (A) a representative microRNA (miR-238), (B) tncRNA (tncR35), and (C) siRNA (Ct1189, from protein-coding gene C44B11.6) in total RNA from the wild type and mutants defective in components of RNAi pathways. Each lane of a 12.5% acrylamide urea gel was loaded with 25 μg of total RNA. Loading was calibrated by hybridizing U6 snRNA probe to each filter after completion of the small RNA analysis. See Table 1 for quantitative analysis.
