TABLE 2.
Translocation rates of 2′-deoxy–substituted tRNAs
| P-site substrate | A-site substrate | kobs (sec−1) |
|---|---|---|
| Translocation rates were measured in polyamine buffer with fivefold excess of untagged EF-G at 25°C and the errors are ±SEM. | ||
| aNative tRNAfMet and tRNAPhe. | ||
| bElongator tRNAMet and tRNAPhe transcripts. | ||
| tRNAfMet a | fMet-Phe-tRNAPhe a | 14.4 ± 0.2 |
| tRNAfMet a | fMet-Phe-tRNAPhe b | 8.3 ± 0.1 |
| tRNAMet b | NAc-Phe-tRNAPhe b | 7.8 ± 0.4 |
| Fragmented tRNAMet | NAc-Phe-tRNAPhe b | 5.3 ± 0.2 |
| 2′-d1 | NAc-Phe-tRNAPhe b | 4.5 ± 0.2 |
| 2′-d2 | NAc-Phe-tRNAPhe b | 5.3 ± 0.6 |
| 2′-d3 | NAc-Phe-tRNAPhe b | 4.3 ± 0.2 |
| 2′-d4 | NAc-Phe-tRNAPhe b | 5.1 ± 0.2 |
| 2′-d5 | NAc-Phe-tRNAPhe b | 4.9 ± 0.7 |
| 2′-d12 | NAc-Phe-tRNAPhe b | 4.9 ± 0.2 |
| 2′-d13 | NAc-Phe-tRNAPhe b | 4.3 ± 0.8 |
