TABLE 1.
Translocation rates of 2′-deoxy–substituted tRNAs and an ASL4Phe from the ribosomal P- and A-sites, respectively
| P-site substrate | kobs (sec−1) |
|---|---|
| Translocation rates were measured in conventional buffer with fivefold excess of C-terminal hexahistidine tagged EF-G at 25°C. | |
| tRNAMet | 0.005 |
| Fragmented tRNAMet | 0.003 |
| 2′-d1 | 0.003 |
| 2′-d2 | 0.003 |
| 2′-d3 | 0.003 |
| 2′-d4 | 0.006 |
| 2′-d5 | 0.004 |
| 2′-d6 | 0.003 |
| 2′-d7 | 0.003 |
| 2′-d8 | 0.005 |
| 2′-d9 | 0.004 |
| 2′-d10 | 0.005 |
| 2′-d11 | 0.005 |
| 2′-d12 | 0.004 |
| 2′-d13 | 0.005 |
| 2′-d14 | 0.004 |
| 2′-d15 | 0.004 |
| 2′-d16 | 0.004 |
| 2′-d17 | 0.005 |
