Ribose 2′-hydroxyl groups in the 5′ strand of the acceptor arm of P-site tRNA are not essential for EF-G catalyzed translocation

Interaction of P- and E-site tRNAs with the 23S rRNA. The contacts observed in the 5.5 Å resolution structure of the 70S ribosome (Yusupov et al. 2001) are displayed using an all-atom model of the ribosome (Tung and Sanbonmatsu 2004). Nucleotides indicated in green and in blue are located in the P- and the E-sites, respectively. Backbone positions between 1–17 in P-tRNA (yellow) and E-tRNA (cyan) that interact with the 23S rRNA are indicated by red spheres. The figure was created using ViewerLite (Acclerys) and rendered with PovRay (http://www.povray.org).