Ribose 2′-hydroxyl groups in the 5′ strand of the acceptor arm of P-site tRNA are not essential for EF-G catalyzed translocation

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FIGURE 4.
FIGURE 4.

Rapid kinetic analysis of translocation. (A) Translocation kinetics of untagged EF-G (trace 1) and C-terminal hexahistidine tagged EF-G (trace 2). (B) Representative traces of single 2′-deoxy–substituted fragmented tRNAMet. Trace 3 (red) indicates translocation of fragmented tRNAMet; trace 4 (green), translocation of 2′-deoxy 1; trace 5 (black), translocation of 2′-deoxy 4. Fluorescence change is in arbitrary units.

This Article

  1. Published in Advance February 17, 2006, doi: 10.1261/rna.2290706 RNA 12: 580-588

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