Ribose 2′-hydroxyl groups in the 5′ strand of the acceptor arm of P-site tRNA are not essential for EF-G catalyzed translocation

Folding of fragmented E. coli tRNA^Met. (A) Secondary structure of tRNA^Met showing the nick in the phosphodiester backbone between positions 17 and 18. (B) Folding of fragmented tRNA^Met analyzed by nondenaturing PAGE. (Lane tRNA^Met) E. coli tRNA^Met transcript, (lane RNA 1–17) unsubstituted smaller RNA fragment, (lane RNA 1–17 (2′-d1)) small RNA fragment with a single 2′-deoxy substitution at position 1. Plus (+) and minus (−) signs indicate presence and absence, respectively, of the 3′ large fragment (positions 18–76). Mobility of folded tRNA^Met, 5′ small fragment, and 3′ large fragment is indicated on the right.